Enzyme concentration
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If the temperature is too high then the enzyme will denature. This means that the active site will be damaged, and will no longer fit with the substrate. This means that no reaction will occur. pH Independent - If the pH is very different to an enzymes optimum pH then the enzyme can be denatured as the hydrogen ions (of which pH is a measure) can interact with the amino acid R groups and affect the way they bond with…...
How different concentrations of the enzyme pectinase affect the degradation of the substrate pectin
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Aim: My aim is to find out how different concentrations of the enzyme pectinase affect the degradation of the substrate pectin, and hence the volume of clarified apple juice produced in a specific duration of time. Hypothesis: Will different enzyme concentrations have different effects on the yield of clarified apple juice obtained from apple pulp of equal masses, in a given time? Biological Knowledge: Enzymes are a class of proteins, which are biological catalysts responsible for speeding up and controlling…...
Enzyme Linked Immunosorbent Assay (ELISA)
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An enzyme linked immunosorbent assay (ELISA) was used to quantify IgM, IgE and IgG, as shown in figures 1 and 2. The optical density measured was the result of a colour change during the assay. This was due to antigens binding to the immobilised antibody coating the ELISA plate wells. This allowed horseradish peroxidase (HRP) conjugated secondary antibodies to also bind to the antigens, which when the chromogenic TMB substrate was added caused a reaction resulting in a colour change…...
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Enzyme Catalysis
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Enzymes are proteins that act as catalysts to regulate metabolism by selectively speeding up chemical reactions in the cell without being consumed during the process. During the catalytic action, the enzyme binds to the substrate – the reactant enzyme acts on – and forms an enzyme-substrate complex to convert the substrate into the product. Each type of enzyme combines with its specific substrate, which is recognized by the shape. In the enzymatic reaction, the initial rate of activity is constant…...
Effect of Hydrogen Peroxide Concentration on Enzyme Catalase
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Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also, they can speed up Metabolic reactions without being changed or used up. During a reaction, an enzyme molecule combines temporarily with the substrate. When the reaction is complete, the enzyme molecules returns to its original dorm and the product is released. So enzymes are never wasted and always recycled. Due to enzymes…...
The Effect of the enzyme Catecholase on the Catalysis of Catechol to Benzoquinone
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Enzymes are defined as proteins that catalyze, or speed up chemical reactions Enzymes work on molecules known as substrates, and change them into different molecules. Enzymes have been used in a variety of ways since their discovery from the making of high-fructose corn syrup by processing starch into sugar (2) to manipulating DNA for use in PCR reactions and tissue browning. Most enzymes are very specific as to which substrates they work on (3). The reason enzymes are specific to…...
Biology: Experiment
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Aim: The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC, which is the body temperature, where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range…...
Lab report on enzyme activity
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Enzymes are a protein serving as a catalyst, a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals, like the different players on a ball team, they have different specific structures and jobs. As one ball player may be very tall and one short, the specific different shape of the active site on an enzyme…...
Mastering Biology Suggested Answer Book
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The rate of reaction increases when the polyphenol oxidase concentration increases. 1m b Rate of reaction = 1/18 = 0.056 1m From the graph, the enzyme concentration is around 1.15%. 1m c Use boiled enzyme in the tube. 1m d The enzyme does not work in acidic medium. / The enzyme is denatured in acidic medium. 1m e The colour change becomes faster at start 0.5m as enzyme activity increases with temperature. 0.5m When temperature becomes too high, no further…...
Effects of pH on Amylase Activity
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Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes, including amylase, function best at a certain optimal pH. Therefore, in this experiment, the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of the body, or 7.0, since that is where the…...
Scientific Investigation of the Peroxidase Enzyme & Temperature
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Abstract In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance, which…...
Factors Affecting the Rate of Enzyme Activity
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The Purpose of doing this experiment is “how factors affect on the rate of enzyme activity.” “We examine the affects of enzyme activity when exposing enzymes to different substrates, PH and temperature. In this experiment a type of enzyme called Peroxides has been used. Peroxidase is the enzymes are found in living organism so, it can be founded in both liver and potato. For measuring the rate of enzyme activity in each experiment the height of the frothy foam column…...
Liver Enzyme Lab Report
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What temperature does the enzyme really work correctly in? (Hypothesis) If the temperature is below 40 but above 20, then the liver will show bubbles. If the temperature level is raised higher than the maximum temperature level, then an extreme decline in enzyme activity would occur following by the fast denaturing of the enzyme, rendering it is permanently useless. Also about 37 ° C is body temperature. The liver that was at 25 ° C had a substantial quantity of…...
Test for enzyme activity Lab
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Purpose: The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity. Hypothesis: I believe that the room temperature activity will produce the most activity. Materials: 3% hydrogen peroxide, a household blender, potatoes (3 medium size potatoes, that are peeled and quartered), tap water, 8 test tubes, 8 transfer pipets, sugar packets, salt, a small saucepan, a refrigerator with a freezer, stove, 4 clear plastic cups, tongs (to remove test…...
Scientific Methodology & Enzyme Activity
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Laboratory 2: Scientific Methodology & Enzyme Activity Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level). Hypothesis: Part a: If different amounts of enzyme solution are added to the hydrogen peroxide, then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions, meaning more oxygen will be produced quicker. Part b: If different temperatures are used…...
Enzyme catalysis lab
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Enzyme catalysis was observed in order to analyze how changes in temperature, pH, enzyme concentration, and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly, decreases, and levels off or completely stops, and can be further affected by environmental factors, which play a crucial role in regulating enzymes and metabolic processes.…...
The Effect of pH on Enzymatic Reaction Rate
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Abstract Enzymes are affected by changes in pH. The most favorable pH value— the point where the enzyme is most active—is known as the optimum pH. This experiment was conducted to determine the effect of pH reaction rate on an enzyme, catalase, from yeast. The experimental results indicate that the catalase worked best at a neutral pH level of seven (7). Introduction An enzyme is a protein molecule that serves as a catalyst. “The basic function of an enzyme is…...
Effect of Enzymes
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Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2), which is a toxic chemical to the human body. When hydrogen peroxide is broken down, the end products are Water (H2O) and Oxygen (O2). In this report, the reaction of catalase to hydrogen peroxide is being tested. Furthermore, the effects of temperature, concentration, and pH level on this reaction are being considered. We hypothesize that he experiments will show that Catalase speeds…...
Enzyme Lab Report
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Enzymes are proteins produced by living cells that act as catalysts, which affect the rate of a biochemical reaction. They allow these complex biochemical reactions to occur at a relatively low temperature and with less energy usage. In enzyme-catalyzed reactions, a substrate, the substance to be acted upon, binds to the active site on an enzyme to form the desired product. Each active site on the enzyme is unique to the substrate it will bind with causing each to have…...
Biology on The Performance of An Enzyme
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Enzymes are considered catalysts; substances that increase the rates of reactions. Enzymes are responsible for thousands of metabolic processes that involve the sustainment of life, one common one is simple food digestion. Without enzymes, digestion would occur too slowly for life to continue. Enzymes maintain a protein structure consisting of one or possibly more than one polypeptide chains of defined primary structure, and take up a characteristic folded form in the native state. Should the structure be modified by an…...
Effect of Temperature on Lipase
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Theory The higher the substrate concentration the more quickly product is produced (rate of reaction increases) until enzyme saturation is reached at which time more substrate has no further effect. Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as potato and liver. It…...
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FAQ about Enzyme

How different concentrations of the enzyme pectinase affect the degradation of the substrate pectin

...I have to be more precise when measuring the volume of pectinase solution and distilled with the syringe, taking care look at the lower meniscus; and doing the same when noting down the result from the measuring cylinder. I ought to rinse the chopped...