Lab Report: Genotype and Health Parameters

Introduction

This comprehensive lab report explores the relationship between genotypes of the T.N.F alpha gene and various health parameters among a group of cases. The health parameters under investigation include Dermatology Life Quality Index (DLQI), systolic and diastolic blood pressure, carotid duplex, triglyceride (TG) levels, high-density lipoprotein (HDL) levels, low-density lipoprotein (LDL) levels, fasting blood sugar (FBS), and correlations with allele concentrations.

Methods

The genotypes of the T.N.F alpha gene were categorized into AA, GG, and GA, with A and G representing alleles.

The following statistical tests were performed: ANOVA and student t-tests to assess differences among genotypes and health parameters.

Results

DLQI Scores

Table 21 presents the relationship between genotypes and DLQI scores. There was a highly significant difference between GA and AA genotypes (p = 0.034), with GA exhibiting the highest mean DLQI score (14.00) compared to AA (6.33). No significant difference was observed between A and non-A genotypes or G and non-G genotypes regarding DLQI scores.

*ANOVA

Systolic Blood Pressure

Table 22 reveals no significant differences between T.N.F alpha genotypes (AA, GG, GA) or A and non-A genotypes or G and non-G genotypes regarding systolic blood pressure.

*ANOVA

Diastolic Blood Pressure

Table 23 shows no significant differences between T.N.F alpha genotypes (AA, GG, GA) or A and non-A genotypes or G and non-G genotypes regarding diastolic blood pressure.

*ANOVA

Carotid Duplex

Table 24 demonstrates no significant differences between T.N.F alpha genotypes (AA, GG, GA) or A and non-A genotypes or G and non-G genotypes regarding carotid duplex.

*ANOVA

Triglyceride (TG) Levels

Table 25 illustrates highly significant differences in TG levels among different T.N.F alpha genotypes. AA genotype had the highest TG levels (343.33) compared to GG (199.03) and GA (216.33) genotypes. A versus non-A genotype showed significant differences (p = 0.0001), with non-A genotypes being protective against high TG levels. No significant differences were found regarding G versus non-G genotype and TG levels.

*ANOVA

High-Density Lipoprotein (HDL) Levels

Table 26 demonstrates significant differences in HDL levels among different T.N.F alpha genotypes. AA genotype had lower HDL levels (31.67) compared to GG (37.57) and GA (30.67) genotypes. A versus non-A genotype showed significant differences (p = 0.0001), with non-A genotypes being protective against low HDL levels. No significant differences were found regarding G versus non-G genotype and HDL levels.

*ANOVA

Cholesterol Levels

Table 27 illustrates significant differences in cholesterol levels among different T.N.F alpha genotypes. AA genotype had higher cholesterol levels (263.33) compared to GG (173.60) and GA (208.33) genotypes. A versus non-A genotype showed significant differences (p = 0.0001), with non-A genotypes being protective against high cholesterol levels. No significant differences were found regarding G versus non-G genotype and cholesterol levels.

*ANOVA

Low-Density Lipoprotein (LDL) Levels

Table 28 illustrates significant differences in LDL levels among different T.N.F alpha genotypes. AA genotype had higher LDL levels (163.00) compared to GG (96.23) and GA (134.40) genotypes. A versus non-A genotype showed significant differences (p = 0.0001), with non-A genotypes being protective against high LDL levels. No significant differences were found regarding G versus non-G genotype and LDL levels.

*ANOVA

Fasting Blood Sugar (FBS)

Table 29 reveals no significant differences in FBS levels among different T.N.F alpha genotypes. However, there was a significant difference between G and non-G genotypes (p = 0.046).

*ANOVA

Correlations

A Allele Concentration and Health Parameters

Tables 30 to 34 provide correlations between A allele concentration and various health parameters. No significant correlations were found between A allele concentration and PASI, DLQI, carotid duplex, systolic blood pressure, diastolic blood pressure, waist circumference, or FBS levels.

G Allele Concentration and Health Parameters

No significant correlations were observed between G allele concentration and PASI, DLQI, carotid duplex, systolic blood pressure, diastolic blood pressure, waist circumference, or FBS levels (Tables 31 to 33).

Discussion

This study indicates significant associations between T.N.F alpha genotypes and various health parameters, including DLQI, TG, HDL, LDL, and cholesterol levels. A alleles appear to be unfavorable in relation to these parameters. However, no correlations were found between A or G allele concentrations and health parameters, suggesting a complex interplay between genetics and health outcomes that requires further investigation.

Conclusion

In summary, this study demonstrates the impact of T.N.F alpha genotypes on several health parameters. A alleles are associated with unfavorable outcomes in DLQI, TG, HDL, LDL, and cholesterol levels. These findings highlight the importance of genetic factors in health and warrant further research to elucidate the underlying mechanisms.