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Passive transport processes, such as diffusion and osmosis, play a crucial role in the movement of molecules across cellular membranes. This lab aims to explore these phenomena and their impact on various biological systems. The experiment involves four parts: Part A investigates the movement of molecules in a dialysis bag; Part B explores osmosis using different solutions; Part C examines water potential in potato cells, and Part D observes the effects of salt on onion cells.
Part A: Dialysis Bag Experiment
Part B: Osmosis with Different Solutions
Part C: Water Potential in Potato Cells
Part D: Effects of Salt on Onion Cells
In Part A, the color change in the bag and the beaker indicates the movement of molecules.
This suggests the occurrence of diffusion and possibly osmosis.
In Part B, changes in the mass of dialysis bags indicate osmotic movement of water. The molarity of solutions in the bags is crucial for understanding the osmotic potential.
In Part C, the change in mass of potato cylinders provides insight into water potential. The comparison between regular and sweet potatoes allows for understanding variations in cellular structures. In Part D, the effects of salt on onion cells demonstrate how osmotic pressure can impact cell morphology.
This lab provides valuable insights into passive transport mechanisms, emphasizing diffusion and osmosis. The observations and calculations help in understanding the principles governing these processes. The variations seen in different parts of the experiment underscore the importance of these mechanisms in maintaining cellular balance.
To enhance the experiment, consider additional variables such as temperature and concentration gradients. Further exploration of different solutes and membrane types could deepen the understanding of passive transport. Additionally, repeating the experiment with variations in time intervals may provide a more comprehensive perspective on the kinetics of diffusion and osmosis.
Future research could focus on the molecular aspects of passive transport, including specific transport proteins involved. Investigating the impact of environmental factors on diffusion and osmosis in various organisms would broaden the applicability of the findings.
Part A. Data table (Diffusion)
Color | Color | Glucose Content | Glucose Content | |
Time | Dialysis Bag | Beaker | Dialysis | Beaker |
Start | Cloudy white | Yellow brown | Yes | No |
30min | Light blue near top, cloudy white at bottom | Cloudy yellow/orange | Yes | Yes |
Part B. Data table (Osmosis)
Solution | Dialysis Bag Initial Mass(g) | Dialysis Bag Final Mass(g) | Change in Mass(g) | % Change in Mass |
Water | 10.4g | 11.9g | 1.5g | 14.4% |
0.2M | 4.8g | 8.1g | 3.3g | 40% |
0.4M | 12.3g | 15.2g | 2.9g | 23.6% |
0.6M | 11.9g | 15.6g | 3.7g | 31.1% |
0.8M | 12.1g | 12.1g | 0g | 0% |
1.0 M | 4.7g | 16.8g | 12.1g | 257.4% |
?M | 11.4g | 15.9g | 4.5g | 39.4% |
Part A:
Part B:
Part C:
Throughout the lab, errors such as weighing with the cover on, incomplete tying of dialysis bags, and insufficient drying may have occurred. Despite these, the data collected is considered reasonably accurate.
Discussion: The lab observed passive transport, disproving the hypothesis about starch diffusion but confirming hypotheses about water movement in parts B and C. The addition of salt to onions showcased cell hypertonicity and subsequent recovery with water.
Lab Report: Understanding Passive Transport - Diffusion and Osmosis. (2024, Feb 27). Retrieved from https://studymoose.com/document/lab-report-understanding-passive-transport-diffusion-and-osmosis
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