Investigating the Effect of Amylase Concentration on Diffusion Rate

Categories: Biology


This laboratory report details an experiment aimed at exploring the impact of different amylase concentration levels on the rate of diffusion using agar jelly blocks infused with starch and iodine. The research hypothesis suggested that higher amylase concentrations would lead to faster diffusion rates. The experiment was conducted with concentrations of 3%, 5%, 10%, and 15% amylase solutions. Data was collected and analyzed, revealing interesting trends and potential outliers.

1. Introduction

In this experiment, we investigated the effects of varying amylase concentration levels on the rate of diffusion.

The key motivation for this study was to gain a deeper understanding of how cells transport materials across their membranes. Diffusion, the movement of molecules from areas of higher concentration to areas of lower concentration, plays a crucial role in this process.

The primary research question was: Does different amylase concentration levels affect the rate of diffusion?

To answer this question, we infused agar jelly cubes with starch and iodine and then soaked them in different amylase concentration solutions (3%, 5%, 10%, and 15%).

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This allowed us to observe how diffusion occurred within cells under varying conditions.

2. Methodology

2.1 Materials

  • Agar jelly cubes infused with starch and iodine
  • Amylase solutions of different concentrations (3%, 5%, 10%, 15%)
  • Sharp objects (e.g., knife)
  • Beakers

2.2 Procedure

  1. Take three samples from each different concentration level of amylase solution.
  2. Record the time it takes for the agar jelly cubes to become completely clear.
  3. Repeat the experiment to ensure reliability and calculate averages and standard errors.

2.3 Safety and Ethical Considerations

  • Follow safety recommendations, including handwashing before and after handling solutions and agar jelly.

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  • Exercise caution when handling sharp objects and glassware to prevent accidents.

3. Results

3.1 Initial Data

Amylase Concentration Sample/Test 1 Sample/Test 2 Sample/Test 3
3% 1 hour 6 mins 42 secs 1 hour 19 mins 57 secs 1 hour 7 mins 26 secs
5% 59.07 mins 1 hour 3 mins 47 secs 1 hour 29 mins 40 secs
10% 51.57 mins 56.32 mins 51.38 mins
15% 56.15 mins NA 1 hour 16 mins 33 secs

3.2 Processed Data - Averages

Amylase Concentration Average Time (minutes)
3% 71.36
5% 70.59
10% 55.53
15% 64.11

4. Analysis and Interpretation of Data

4.1 Data Trends

The data reveals that the highest average time for diffusion occurred with the 3% amylase concentration solution, suggesting that weaker concentrations led to longer diffusion times. A negative linear relationship is observed between amylase concentration and diffusion time.

5. Evaluation of Methodology

The experimental process provided relatively reliable and valid data, with a few potential outliers. One significant source of error was observed in the 10% amylase concentration solution, possibly due to a lab error or inaccurate measurement of the solution's concentration.

To improve future experiments, increasing the sample size, conducting multiple trials, and careful handling of solutions are recommended.

6. Conclusion

In conclusion, the experiment suggests that as amylase concentration levels increase, the time for diffusion decreases, in line with our hypothesis. However, the presence of outliers and potential errors in the 10% concentration solution indicate the need for further investigation. This experiment provides valuable insights into the impact of amylase concentration on diffusion rates, contributing to our understanding of cellular processes.

7. Reference List

  1. Khan Academy. (2015). Passive Transport. Retrieved from
  2. (2019). Practical for Agar Block Diffusion with Acid | Year 11 SACE - Biology. Retrieved from
Updated: Jan 09, 2024
Cite this page

Investigating the Effect of Amylase Concentration on Diffusion Rate. (2024, Jan 06). Retrieved from

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