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L-Cysteine, a semi-essential amino acid, plays a crucial role in various biological processes and is used in alternative medicine for treating multiple health conditions. This study investigates the influence of The Trivedi Effect® - Consciousness Energy Healing Treatment on the physicochemical and thermal properties of L-cysteine. The L-cysteine sample was divided into control and treated groups, with the treated group receiving remote biofield energy healing by Dahryn Trivedi. The results revealed significant alterations in particle size distribution, surface area, crystalline properties, and thermal characteristics of the treated L-cysteine sample compared to the control.
This suggests the potential for improved solubility, bioavailability, and thermal stability of biofield energy-treated L-cysteine.
L-Cysteine is considered a semi-essential amino acid, primarily found in the extracellular space as L-cystine. While the human body can biosynthesize it to some extent under normal conditions, it also relies on dietary sources of sulfur, making it an essential nutrient.
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Intracellularly, L-cysteine plays a vital role in cellular homeostasis, protein synthesis, and the production of glutathione, hydrogen sulfide, and taurine. Additionally, it is used in alternative medicine to address various health issues such as cardiovascular disease, inflammation, angina, chronic bronchitis, diabetes, osteoarthritis, and influenza. Furthermore, L-cysteine supports lung health, particularly in chronic obstructive pulmonary disease (COPD) patients. Dietary sources of L-cysteine include dairy products, meat, eggs, legumes, oats, and various vegetables. Some studies even suggest its potential in colon cancer prevention, detoxification, and enhancing sports performance.
L-cysteine deficiency can occur in certain populations, including the elderly, infants unable to biosynthesize it, and individuals with metabolic syndrome or malabsorption issues. A deficiency may lead to slow recovery from injuries and compromised immune function. As a result, healthcare professionals provide L-cysteine in pharmaceutical or nutraceutical formulations to meet the body's needs.
Physicochemical properties significantly influence the biological performance of amino acids like L-cysteine. Researchers have focused on altering these properties, including particle size, surface area, crystalline structure, and melting point, to enhance amino acid performance.
In recent years, Biofield Energy Treatment has gained attention for its potential to enhance the properties of various compounds due to its significant effects on living and non-living objects. Biofield Energy is a form of energy medicine utilized in Complementary and Alternative Medicine (CAM), encompassing both traditional and contemporary approaches to address various health conditions. Living organisms possess a unique energy field known as the Biofield Energy, an infinite and para-dimensional electromagnetic field. The Trivedi Effect®, a natural phenomenon, allows individuals to harness this intelligent energy and transfer it worldwide through the mediation of neutrinos. Consciousness Energy Healing Treatment has demonstrated remarkable effects in various fields, including biotechnology, agriculture science, human health, microbiology, livestock, metals, and ceramics. Scientific evidence also supports the significant impact of the Trivedi Effect® on the physicochemical and thermal properties of pharmaceutical, nutraceutical, and organic compounds.
This study aims to investigate the influence of Consciousness Energy Healing Treatment on the physicochemical and thermal properties of L-cysteine. Analytical techniques such as Particle Size Analysis (PSA), Powder X-ray Diffraction (PXRD), Differential Scanning Calorimetry (DSC), and Thermogravimetric Analysis (TGA)/Differential Thermogravimetric Analysis (DTG) were employed to assess any alterations.
L-cysteine was procured from Alfa Aesar, USA. All other chemicals used in the experiments were of analytical grade and obtained from sources in India.
In this study, L-cysteine served as the test compound and was divided into control and Biofield Energy Treated groups. The control sample remained untreated, while the treated sample underwent remote Consciousness Energy Healing Treatment administered by the renowned Biofield Energy Healer, Dahryn Trivedi (USA). The treatment process involved placing the sample under standard laboratory conditions and subjecting it to the Trivedi Effect® - Consciousness Energy Healing Treatment for 3 minutes through a Unique Energy Transmission process. Additionally, the control sample underwent a "sham" treatment by an individual in similar laboratory conditions, who had no knowledge of the Biofield Energy Treatment. Following the treatment, both the control and Biofield Energy Treated samples were sealed and characterized using modern analytical techniques.
The physicochemical and thermal analysis of L-cysteine was conducted using the following techniques:
Equation 1:
G = kλ/βcosθ (1)
Where:
The percent change in specific surface area, particle size, crystallite size, peak intensity, melting point, latent heat, weight loss, and maximum thermal degradation temperature of Biofield Energy Treated L-cysteine was calculated in comparison to the control sample using Equation 2.
Equation 2:
Percentage Change = [(Treated Value - Control Value) / Control Value] x 100
Particle size analysis was employed to assess the impact of Biofield Energy Treatment on the particle size distribution of L-cysteine samples compared to the control. The results revealed a significant decrease in particle size distribution (d10, d50, d90, and D(4, 3)) of Biofield Energy Treated L-cysteine by 10.18%, 12.97%, 16.83%, and 14.36%, respectively, compared to the control (Table 1). The increased specific surface area of the Biofield Energy Treated sample by 14.29% compared to the control L-cysteine sample is noteworthy. Previous scientific studies have established a significant correlation between surface area and solubility. Reduced particle size enhances the effective surface area available for dissolution, thereby improving bioavailability within the body. Consequently, Biofield Energy Treated L-cysteine is anticipated to exhibit enhanced dissolution and bioavailability profiles compared to the control sample.
Figure 1 displays the diffractograms obtained from powder X-ray diffraction (PXRD) analysis of both the control and Biofield Energy Treated samples. Sharp and intense peaks were evident in both diffractograms, confirming the crystalline nature of the Biofield Energy Treated sample, similar to the control sample. However, notable differences were observed in peak positions, intensities, and the presence of characteristic peaks.
In the Biofield Energy Treated sample, the highest peak intensity was observed at 2θ = 23.05°, while in the control sample, it was at 2θ = 24.67°. Furthermore, several peaks at 2θ = 13.24º, 33.84º, and 40.04º present in the control sample were absent in the Biofield Energy Treated sample. Instead, new peaks emerged at 2θ = 23.05º, 43.42º, and 49.81º in the treated sample. The Biofield Energy Treated sample exhibited significant changes in peak intensities and crystallite sizes, with alterations ranging from -47.62% to 110.11% and -17.74% to -74.57%, respectively, compared to the control sample.
Notably, the average crystallite size of the Biofield Energy Treated sample was significantly reduced by 49.81% (450.75 nm) compared to the control sample (898 nm). Such alterations in peak positions, intensities, and crystallite sizes suggest potential changes in the crystalline properties of L-cysteine induced by the Biofield Energy Treatment. These observations, including the emergence of new peaks and the absence of characteristic peaks, hint at the possible formation of new polymorphs of L-cysteine after the Biofield Energy Treatment. Previous studies have shown that modifications in the crystalline structure can impact drug bioavailability and efficacy. Thus, it is plausible that Biofield Energy Treated L-cysteine may exhibit enhanced bioavailability and efficacy compared to the control sample.
Figure 2 illustrates the differential scanning calorimetry (DSC) thermograms of both the control and Biofield Energy Treated samples, allowing us to assess differences in their melting temperature and latent heat. Previous studies on L-cysteine have reported its decomposition during heating rather than sublimation, as indicated by peaks coinciding with the drop in the thermogravimetric analysis (TGA) thermogram.
Both samples exhibited two endothermic peaks during thermal heating. Although the peak temperatures in the Biofield Energy Treated sample showed minor changes, the latent heat (ΔHdecomposition) corresponding to both peaks significantly differed from that of the control sample. Specifically, the treated sample exhibited a slight reduction in peak temperature (0.20% and 0.40%) for the first and second peaks, respectively. However, the ΔHdecomposition was significantly altered, with increases of 11.45% and 20.79% for the first and second peaks, respectively, compared to the control sample (Table 3).
These DSC results indicate that the decomposition temperatures of the Biofield Energy Treated sample remained similar to those of the control sample. However, the increased latent heat at those temperatures suggests potential alterations in the crystal structure and molecular arrangement of L-cysteine after the Biofield Energy Treatment. Consequently, the thermal decomposition pattern of the Biofield Energy Treated sample differs from that of the control L-cysteine sample due to changes in latent heat.
The thermal gravimetric analysis (TGA) thermograms of both the control and treated samples revealed significant weight loss during thermal degradation. The degradation pattern of both samples during heating was consistent with previous scientific studies. Further analysis of the thermograms indicated a notable reduction in weight loss for the treated sample compared to the control sample.
The treated sample exhibited a 93.94% weight loss during thermal heating, representing a 5.59% reduction compared to the total weight loss of the control sample (99.50%). Consequently, the resulting residue weight of the treated sample significantly increased by 1111.20% compared to the control L-cysteine sample.
Additionally, the differential thermogravimetric analysis (DTG) results (Table 4) indicated a slight change in the maximum thermal degradation temperature (Tmax) of Biofield Energy Treated L-cysteine compared to the control sample. The Tmax of the control sample was observed at 221.06ºC, while the treated sample exhibited a slightly lower Tmax, reduced by 0.50% to 219.96 ºC.
Collectively, the TGA/DTG studies suggest increased thermal stability of the Biofield Energy Treated sample compared to the control L-cysteine sample. These findings provide insights into the alterations in the physicochemical and thermal properties of L-cysteine induced by the Trivedi Effect®-Consciousness Energy Healing Treatment.
The particle size analysis revealed significant changes in the particle size distribution of the Biofield Energy Treated L-cysteine sample compared to the control sample. Specifically, the treated sample exhibited a reduction in particle sizes by 10.18% (d10), 12.97% (d50), 16.83% (d90), and 14.36% [D(4, 3)] in comparison to the control sample. Consequently, the surface area of the treated sample increased by 14.29% compared to the control L-cysteine sample, indicating a potential improvement in solubility and bioavailability.
The PXRD analysis showed alterations in the intensities and crystallite sizes of the Biofield Energy Treated L-cysteine sample compared to the control sample. Peak intensities exhibited changes ranging from -47.62% to 110.11%, and crystallite sizes showed variations from -17.74% to -74.57%. The average crystallite size of the treated sample was significantly reduced by 49.81% compared to the control sample. These results suggest that the Biofield Energy Treatment may have induced new polymorphic forms of L-cysteine, potentially impacting its bioavailability and efficacy.
The DSC analysis indicated alterations in the thermal properties of the Biofield Energy Treated L-cysteine sample. While the peak temperatures remained similar between the two samples, the latent heat of decomposition (ΔHdecomposition) showed significant changes. The treated sample exhibited an 11.45% increase in ΔHdecomposition for the first peak and a 20.79% increase for the second peak compared to the control sample. These findings suggest that the Biofield Energy Treatment may have affected the crystalline structure and molecular patterns of L-cysteine, influencing its thermal behavior.
The TGA/DTG analysis demonstrated a significant reduction in the weight loss of the Biofield Energy Treated L-cysteine sample during thermal degradation compared to the control sample. The treated sample exhibited a 5.59% decrease in total weight loss and a remarkable 1111.20% increase in residue weight. Additionally, the maximum thermal degradation temperature (Tmax) of the treated sample showed a slight reduction of 0.50% compared to the control sample, indicating increased thermal stability.
The results of this study reveal that the Trivedi Effect®-Consciousness Energy Healing Treatment has a profound influence on the physicochemical and thermal properties of L-cysteine. The treated sample exhibited significant changes in particle size distribution, surface area, crystalline properties, and thermal stability compared to the control sample. These alterations suggest that Biofield Energy Treated L-cysteine may have enhanced solubility, bioavailability, and thermal stability, potentially making it a more effective compound for various applications. Further research is warranted to explore the practical implications of these findings in pharmaceutical and nutraceutical formulations.
In conclusion, this study demonstrates that the Trivedi Effect®-Consciousness Energy Healing Treatment has a profound influence on the physicochemical and thermal properties of L-cysteine. The treated sample exhibited significant changes in particle size distribution, surface area, crystalline properties, and thermal stability compared to the control sample. These alterations suggest that Biofield Energy Treated L-cysteine may have enhanced solubility, bioavailability, and thermal stability, potentially making it a more effective compound for various applications. Further research is warranted to explore the practical implications of these findings in pharmaceutical and nutraceutical formulations.
Impact of The Trivedi Effect® on L-Cysteine. (2024, Jan 17). Retrieved from https://studymoose.com/document/impact-of-the-trivedi-effect-on-l-cysteine
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