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This comprehensive lab report presents the results and analysis of a genetic association study conducted on 307 healthy adult participants. The study aimed to investigate the relationship between the CRP rs3091244 single nucleotide polymorphism (SNP), cognitive performance, and MCP-1 plasma levels. The research included genotyping, statistical analysis, and stratification by covariates.
Genetic association studies play a crucial role in understanding how genetic variations can impact various traits and health outcomes. In this study, we focused on the CRP rs3091244 single nucleotide polymorphism (SNP) and its potential associations with cognitive performance and MCP-1 plasma levels in a cohort of 307 healthy adult participants.
Genotyping was conducted on 307 participants to determine their genetic makeup regarding the CRP rs3091244 SNP.
The results showed that there were 164 T-allele carriers and 143 CC-homozygotes. Interestingly, the analysis revealed a departure from the Hardy-Weinberg equilibrium (X2 (2) = 12.836, P = .002). This departure may be attributed to the unequal distribution of nationalities among the participants, as specific nationalities might have different prevalence rates for this particular SNP.
To assess the normality of various characteristics, the Shapiro-Wilk test was applied.
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The results indicated that most characteristics, including BMI, MMSE score, sex, and more, were non-normally distributed (P < .001). However, height (P = .253) and body mass (P = .069) appeared to follow a normal distribution.
The Pearson Chi-Squared goodness of fit test was employed to investigate differences in the distribution of characteristics between genotype groups. Surprisingly, there were no significant differences in distribution observed for any of the examined categories, including sex, age, current smoker status, excessive alcohol consumption, education level, and living alone status (all P > .05).
It is noteworthy that there were no significant differences in age, sex, health, lifestyle habits, or prior cognitive well-being among the participants.
This homogeneity within the cohort enhances the validity of the study's findings.
Mann-Whitney-U tests were conducted to assess the differences in MCP-1 plasma levels and cognitive performance between genotype groups. The results unveiled several significant findings:
| Outcome Measure | Genotype Group | Median | Interquartile Range | P-Value |
|---|---|---|---|---|
| Plasma MCP-1 Levels (pg/mL) | T-allele Carriers | 190.035 | 156.377 – 250.804 | 0.010 |
| CC-homozygotes | 163.000 | 142.043 – 201.617 | ||
| Episodic Memory Test Scores (Z scores) | T-allele Carriers | -0.953 | -3.117 – 0.129 | 0.024 |
| CC-homozygotes | -0.231673 | -2.035 – 0.490 |
These results suggest that T-allele carriers had significantly higher MCP-1 plasma levels (median: 190.035, 156.377 – 250.804 pg/mL) compared to CC-homozygotes (median: 163.000, 142.043 – 201.617 pg/mL). Additionally, a significant difference in episodic memory test scores was observed, with T-allele carriers achieving lower Z scores (median: -0.953, -3.117 – 0.129) compared to CC-homozygotes (median: -0.231673, -2.035 – 0.490).
Our genotyping analysis revealed that out of 307 healthy adults, 164 individuals carried the T-allele, while 143 were CC-homozygotes for the CRP rs3091244 SNP. However, the departure from Hardy-Weinberg equilibrium (X2 (2) = 12.836, P = .002) raised questions about the genetic distribution within our cohort. The deviation could be attributed to the unequal distribution of nationalities among participants, potentially leading to varying prevalence rates of the T-allele within different genetic pools.
Normality testing using the Shapiro-Wilk test demonstrated that most characteristics in our study were non-normally distributed (P < .001), except for height (P = .253) and body mass (P = .069).
The Pearson Chi-Squared goodness of fit test indicated that there were no significant differences in the distribution of various characteristics between genotype groups. These characteristics included sex, age, current smoker status, excessive alcohol consumption, education level, and living alone status (all P > .05).
Overall, our analysis did not reveal any significant differences within the cohort concerning age, sex, health, lifestyle habits, or prior cognitive well-being. This consistency strengthens the reliability of our findings.
After controlling for age and sex, significant differences persisted in both plasma MCP-1 levels and episodic memory scores between genotype groups. Particularly, these differences were prominent in the older male participants.
Our study's findings suggest a potential genetic association between the CRP rs3091244 SNP and MCP-1 plasma levels, as well as episodic memory performance. Notably, T-allele carriers exhibited elevated MCP-1 levels and poorer episodic memory compared to CC-homozygotes. This association is of particular interest in the context of cognitive function, as MCP-1 is known to play a role in neuroinflammation and neurodegenerative diseases.
The departure from Hardy-Weinberg equilibrium raises questions about the genetic distribution in our study. The unequal representation of nationalities within the cohort may have influenced the prevalence of specific genotypes, warranting further investigation into the population-specific effects of this SNP.
In conclusion, our genetic association study provides valuable insights into the potential role of the CRP rs3091244 SNP in influencing MCP-1 plasma levels and episodic memory performance. However, further research is needed to elucidate the underlying biological mechanisms and the clinical implications of these associations. Additionally, investigating the population-specific effects of this SNP could offer a more comprehensive understanding of its impact on human health.
Genetic Study: CRP rs3091244 SNP, MCP-1 Levels, and Cognition. (2024, Jan 18). Retrieved from https://studymoose.com/document/genetic-study-crp-rs3091244-snp-mcp-1-levels-and-cognition
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