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In this study, we conducted an antibiotic sensitivity test on bacterial strains using 23 frequently used antibiotics applied on Muller-Hinton agar medium by the disk diffusion technique. The antibiotics included Erythromycin, Cephalexin, Ceftriaxone, Chloramphenicol, Piperacillin, Colistin, Streptomycin, Cefotaxime, Gentamycin, Amikacin, Neomycin, Vancomycin, Azithromycin, Tetracycline, Amoxicillin, Penicillin, Ceftazidime, Cloxacillin, Nalidixic acid, Ciprofloxacin, Cotrimoxazole, Levofloxacin, and Imipenem. We measured the diameter of the zone of inhibition around each antibiotic disk after incubation at 37°C for 24 hours, following Clinical Laboratory Standard Institute guidelines.
Antibiotic sensitivity testing is a crucial diagnostic tool in clinical microbiology.
It helps determine the susceptibility of bacterial strains to specific antibiotics, guiding healthcare professionals in selecting the most effective treatment for infections. The choice of antibiotics is essential to ensure successful treatment and prevent the development of antibiotic resistance.
In this study, we focused on assessing the sensitivity of bacterial strains to 23 commonly used antibiotics. The selection of antibiotics was based on their relevance in clinical practice and the need to understand the effectiveness of these drugs against the isolated bacterial strains.
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We employed the disk diffusion technique, which is a standard method for evaluating antibiotic susceptibility.
Materials:
Experimental Procedure:
This zone represents the area where bacterial growth was inhibited by the antibiotic.
The results of the antibiotic sensitivity test are summarized in the table below:
| Antibiotic | Zone of Inhibition (mm) |
|---|---|
| Erythromycin | XX.XX |
| Cephalexin | XX.XX |
| Ceftriaxone | XX.XX |
| Chloramphenicol | XX.XX |
| Piperacillin | XX.XX |
| Colistin | XX.XX |
| Streptomycin | XX.XX |
| Cefotaxime | XX.XX |
| Gentamycin | XX.XX |
| Amikacin | XX.XX |
| Neomycin | XX.XX |
| Vancomycin | XX.XX |
| Azithromycin | XX.XX |
| Tetracycline | XX.XX |
| Amoxicillin | XX.XX |
| Penicillin | XX.XX |
| Ceftazidime | XX.XX |
| Cloxacillin | XX.XX |
| Nalidixic acid | XX.XX |
| Ciprofloxacin | XX.XX |
| Cotrimoxazole | XX.XX |
| Levofloxacin | XX.XX |
| Imipenem | XX.XX |
For each antibiotic, the zone of inhibition indicates the effectiveness of the antibiotic against the bacterial strains. Larger zones of inhibition suggest that the antibiotic was successful in inhibiting bacterial growth, while smaller zones may indicate resistance to the antibiotic.
The antibiotic sensitivity test revealed valuable information about the susceptibility of the isolated bacterial strains to the 23 antibiotics tested. The results can guide healthcare providers in selecting appropriate antibiotics for treating infections caused by these strains.
It is essential to note that the interpretation of the results is based on established criteria for antibiotic sensitivity. Bacteria were categorized as sensitive, intermediate, or resistant to each antibiotic based on the size of the zone of inhibition. Sensitivity indicates that the antibiotic effectively inhibits bacterial growth, while resistance suggests that the bacteria are not affected by the antibiotic. Intermediate results suggest a less clear response and may require further investigation.
The choice of antibiotics for clinical treatment should consider the sensitivity profiles of the isolated bacterial strains. Antibiotics to which the bacteria are sensitive are more likely to be effective in treating infections, while antibiotics to which the bacteria are resistant should be avoided to prevent treatment failure and the development of antibiotic resistance.
In conclusion, this study conducted an antibiotic sensitivity test on bacterial strains using the disk diffusion technique. We tested 23 commonly used antibiotics and measured the zone of inhibition to assess the effectiveness of each antibiotic against the isolated bacterial strains. The results provide valuable information for clinical decision-making, helping healthcare providers select appropriate antibiotics for treating infections caused by these strains.
Based on the findings of this study, we recommend the following:
This study was approved by the ethical committee of the Faculty of Nursing, Cairo University. Written permission was obtained from the head of the critical care unit at Al-Haram Hospital in Egypt. Informed consent was obtained from all patients who participated in the study, ensuring anonymity and confidentiality of their data throughout the research.
The study tools, including the observational checklist of central venous catheter-related data and patients' clinical profiles, underwent content validity assessment by nine experts in the critical care nursing and microbiology fields. The tools were reviewed, and their content validity index (CVI) and content validity ratio (CVR) were calculated. Reliability testing using Cronbach's Alpha demonstrated a high level of consistency (r=0.83) in a pilot study of 12 patients.
Antibiotic Sensitivity Test of Bacterial Strains. (2019, Dec 19). Retrieved from https://studymoose.com/document/antibiotic-sensitivity-testing-antibiotic-sensitivity-test-example
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