The effect of Catalase Essay
The effect of Catalase
Simultaneously the Hydrogen peroxide will be added, the stopper placed on the boiling tube and then stop watch started. We will then measure the amount of gas produced every 10 seconds, recording our results up to and including 200 seconds. We will then change the temperature of the water bath to varying temperatures listed in the results table and repeat the experiment. Results Time (seconds) Experiment 1 – 17i?? C Experiment 2 – 38i?? C Experiment 3 – 13i?? C Experiment 4 – 60i?? C Experiment 5 – 48i?? C Analysis of Graph The graph is made up of several curves, which start at the origin.
This tells us that the volume of gas produced (rate of reaction) is not directly proportional to time. Here is a summary of what our graph shows: The points make an obvious pattern. The graph shows that volume of gas produced increases as time goes by up until a certain time at which it stops reacting. From the graph we can see one or two very small anomalies but they are not off by much. This could be due to the apparatus and procedure methods. Using more accurate equipment and taking more care when preparing the experiment could reduce these anomalies.
Another source of error is the shaking of the test tube, if all the experiments were not shaken the same amount at the same time the graphs could be off. Conclusion “I predict that as temperature increases so will the rate of the reaction (up to a certain temperature, at which it will no longer ‘function’)” As shown in my results table, and more clearly my graph, you can see what I found supported my prediction mentioned above. However, the temperature at which I predicted that the yeast would not function was off. We operated the experiment at two extremes temperatures, 13i?? C and 60i?? C.
At these temperatures there was a change in the rate of reaction, however, this change was not as much as I predicted. The gap between the most gas produced and the least is only 10cmi??. What is most surprising is that the enzyme reacted quite well at 60i?? C, at this point I would have thought it would have denatured. It also didn’t react as slow as I thought it would at 13i?? C which is also surprising. In my background information I included that the optimum temperature for yeast would be around 37i?? C, however, after the experiment it seems the optimum temperature would be somewhere between 50i??
C and 60i?? C. Evaluation Although the experiment was reasonably accurate, some of the results we took are bound to be off, and as you can see from my graph some of them are. However, they are not off by much at all. To test my results for lack of accuracy I did the first experiment twice and compared those results. From this I found that the end amount of gas produced was 5cmi?? more than it was the second time I did the experiment. Errors in the experiment can be due to several factors. These factors are the ‘key’ factors mentioned above.
These key factors are factors that could change the results by varying amounts if they are not constant. These factors are basically: Time Temperature Amount of Hydrogen Peroxide used pH of the solution Shaking of the test tube We tried to keep these values at a constant and tried to repeat each experiment exactly the same as the last one. However, in experiments where readings are required to be read off a scale there will be a lot of human error. Improving the Experiment There are only a few ways in which we could improve the experiment, and it is likely the highest sources of error came from human error.
However, we could always increase the accuracy of the results by using more accurate equipment, such as an Electro thermostatically controlled water baths which are precise to 0. 05i?? C. Gas syringes would also be a possibility to us. Whether gas syringes would be more accurate or not is not sure, but the scales might be easier to read and therefore reduce human error. Show preview only The above preview is unformatted text This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.
University/College: University of Arkansas System
Type of paper: Thesis/Dissertation Chapter
Date: 6 October 2017