Now Accepting Apple Pay

Apple Pay is the easiest and most secure way to pay on StudyMoose in Safari.

MicroRNAs and Fundamental Cellular Procedures

MicroRNAs (miRNAs), a class of endogenous noncodesmall molecules (18-22nt), play important roles as significant regulators to regulate fundamental cellular procedures via the modulation toward the expression of target genes that associated with a wide range of biological processes, such as cell proliferation, apoptosis and death. Research evidence has shown that miRNA is a potential class of biomarker candidate for cancer classification, early disease diagnosis, especially human cancers, neurological diseases, viral infections and diabetes. So far, there are many kinds of biosensors for microRNA detection that have been reported.

For example, as illustrated in Fig. 19, Zhang and co-worker constructed an off?on switching of a dual amplified electrochemiluminescence (ECL) biosensor based on Pb2+-induced DNAzyme-assisted target recycling and rolling circle amplification (RCA) for microRNA (miRNA) detection. In this study, a dual amplified ECL biosensor by coupling Pb2+-requiring DNAzyme-assisted target recycling amplification with Y junction has been developed for highly sensitive miRNA detection in an off?on manner for the first time.

Get quality help now
Bella Hamilton
Verified writer

Proficient in: Biology

5 (234)

“ Very organized ,I enjoyed and Loved every bit of our professional interaction ”

+84 relevant experts are online
Hire writer

Herein, the “switch-off” sensing platform was obtained by the quenching effect of dopamine towards a luminol/H2O2 system owing to the DNA hybridization reaction of the dopamine modified DNA sequence (S1) with HP hairpin probe. At first, the primer probe was incubated with the assistant probe and the target RNA to form the Y junction structure which was cleaved with the addition of Pb2+ to release miRNA. The released miRNA could initiate the next recycling process, leading to the generation of abundant intermediate DNA sequences (S2). Afterward, the treated glassy carbon electrode (GCE) was electrodeposited with HAuCl4, which presented an outstanding Au nanoparticles platform to modify numerous hairpin probe (HP) through Au?S bonding.

Get to Know The Price Estimate For Your Paper
Number of pages
Email Invalid email

By clicking “Check Writers’ Offers”, you agree to our terms of service and privacy policy. We’ll occasionally send you promo and account related email

"You must agree to out terms of services and privacy policy"
Check writers' offers

You won’t be charged yet!

Then, dopamine (DA) – modified DNA sequence (S1) was applied to unfold the hairpin loop of HP DNA and hybridize with HP hairpin probe, which switching off the sensing system. Due to the quenching effect of DA toward luminol, the ECL signal was reduced which indicated that the system presented the OFF state.

Then, S2 produced by the target recycling process was loaded onto the prepared electrode to displace S1 and served as an initiator for RCA, where numerous repeated DNA sequences coupling with hemin to form a hemin/G-quadruplex exhibited strongly catalytic effect toward H2O2, which could amplified the ECL signal to obtain the second “switch-on” state of the sensing system.

Cite this page

MicroRNAs and Fundamental Cellular Procedures. (2019, Dec 12). Retrieved from

👋 Hi! I’m your smart assistant Amy!

Don’t know where to start? Type your requirements and I’ll connect you to an academic expert within 3 minutes.

get help with your assignment