Concentration of enzymes Essay
Concentration of enzymes
Based on the results obtained from the experiment it can be concluded that the concentration of enzymes influences the rate of a chemical reaction. If enzyme concentration is decreased then the reaction rate will also decrease. If there is sufficient enzyme to bind with substrate then the reaction will proceed fast and if there are insufficient enzymes present then the reaction will slow down
It was predicted that with the increase of concentration of amylase solution, the time took to break down starch would decrease . This was proved correct , if basing on just these trials . The graph shows how with every single increase of the concentration , the amount of drops until the mixture fails to give a blue-black colour with iodine solution disappear decreased. This continually happened , without any outliers. The trend-line of the graph shows the predicted linear line in the relationship between concentration of amylase solution and the amount of drops until the mixture fails to give a blue-black colour with iodine solution. The amount of drops until the mixture fails to give a blue-black colour with iodine solution with a amylase concentration of 0.1% was found to be 12 drops, which was plotted on the graph.
It can be presumed that a higher concentration of amylase is better because it does not take much time to break down the polysaccharide chains and a higher concentration helps to speed up this process. The amylase concentration of 0.1% took only 12 drops until the mixture fails to give a blue-black colour, while the amylase concentration of 0.05% took 21 drops , the amylase concentration of 0.025% took 24 drops and the amylase concentration of 0.01% took 28 drops. Because the purpose of an enzyme is to speed up a reaction, it is logical that amylase would have a higher concentration so it can take less time for it to do its purpose. At higher amylase concentration the time taken until the mixture fails to give a blue-black colour with iodine solution. Despite this, there are sources of error that need to be acknowledge. The trial proved difficult for some, sometimes yielding no results. Another source may be the mixing of the concentration.