Carbohydrate Fermentation

Fermentation is the production of ATP which occurs in the absence of oxygen. For this experiment, S. cerevisiae as well as S. epidermidis were utilized. My S. cerevisiae experiment was noted to have fermented all three carbohydrates used: glucose(AG), fructose(AG), and Mannitol(-). With my experiment of S. cerevisiae: glucose(AG), fructose(AG), mannitol(A)

Beyond twenty-four hours of fermentation, the test tubes could change color altering the results of the experiment. The reading could possibly show a negative response when it was truly positive to begin with.

Phenyl red is added to the test tube and functions as a pH indicator. It will turn a pink or fuchsia color if an alkaline setting is present and yellow in an acidic environment. The addition of this chemical is also used as a medium to determine gram-negative enteric bacteria. If the pH of a solution is 6.8 or below, the phenol red will turn the solution yellow. If the pH of the solution is 7.

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4 or higher, it will turn a pink/fuchsia color.

If the solution turns yellow, that states an acid end product formed which indicates the bacterium utilized the carbohydrate. If the carbohydrate was not used the end product will be ammonia which means the solution has turned yellow. Bacteria contain DNA which is coded for certain enzymes which the organism contains. Numerous carbohydrates can be fermented, but not all bacteria can ferment every single one. In order for a particular type of organism to ferment a specific carbohydrate, it needs an accurate enzyme built inside in order to convert a specific carbohydrate.

Reference

Hands-on-Labs. (2012). A Laboratory Manual of Small-Scale Experiments for the Independent Study of Microbiology. Englewood, CO. Available from www.labpaq.com

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