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Staining Essay Topics & Paper Examples

Fermentation Lab Report

In this experiment milk was fermented into Kefir, then a series of tests were used to confirm that fermentation actually occurred. The tests used to confirm the fermentation were a gas production test, pH test, Gram stain, and turbidity test. The results showed during the conversion of milk to Kefir there was gas produced, a decrease in pH, more bacteria present, and an increase in absorbency. The results proved that fermentation occurred with three positive confirmatory results from the gas production, pH, and turbidity tests, while the Gram stain test acted as a negative confirmatory test. Introduction: The main goal of this experiment was to successfully ferment milk into a yogurt-like product called Kefir. The fermentation is accomplished with a…

Microbiology Laboratory Report

Objectives Aim of this experiment is to differentiate between the two major categories of bacteria , gram positive and gram negative. Through this experiment, gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result, Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour , while Staphylococcus aureus ( S.aureus ) is a positive gram bacteria which stain purple colour. Materials Bacteria : Escherichia coli , Staphylococcus aureus Crystal Violet Gram’s iodine Absolute alcohol Safranin Methodology As per manual. Discussion Gram staining is a very importance preliminary step to differentiate the bacteria into gram positive and gram negative and also enable the bacteria to be examined using a light microscope . Bacteria…

Lab Report about Simple Staining of Microbes

This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion, different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify the basic shape and size of the microbe. In this experiment, the microbes E. coli, Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. These staining techniques are the correct way…

Differential Staining

Through the process of differential staining, there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria, the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan thereby creating very thick cell membranes which causes the cell wall to take up large quantities of basic dye and appears purple. (Hands-on-Labs. (2012)). Conversely, gram-negative bacteria cell walls are much thinner with an outer cell membrane composed of phospholipids and only 10-20% peptidoglycan. Therefore, it appears pink via the process of differential staining. The difference in color can be attributed to the thinner cell wall and the decolorization process that occurs with…