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Physio Ex 9.0 Exercise 12 Activity 1 Essay

Exercise 12: Serological Testing: Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following has an epitope? You correctly answered: b. an antigen

2. Chlamydia trachomatis is
You correctly answered: c. a bacterium that reproduces inside its host cell.

3. The infectious form of Chlamydia that is spread from person to person is the You correctly answered: d. elementary body that stains green in this activity

4. The goal of the direct fluorescent antibody test is to test for the presence of You correctly answered: c. antigen.

Experiment Results

Stop & Think Questions:
The source of the patient samples is
You correctly answered: c. epithelial scrapings
What is the name for the specific part of the antigen that the antibody binds to? You correctly answered: b. epitope

15. Drag the first slide (patient A) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal. You answered: 0 elementary bodies

16. Drag the next slide (patient B) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal. You answered: 15 elementary bodies

17. Drag the next slide (patient C) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal. You answered: 1 elementary bodies

18. Drag the next slide (positive control) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal. You answered: 19 elementary bodies

19. Drag the last slide (negative control) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal. You answered: 0 elementary bodies

 Post-lab Quiz Results
You scored 100% by answering 5 out of 5 questions correctly. 1. In the serology test just performed, the fluorescence is found on the You correctly answered: c. antibody
2. What would most likely happen if you forgot to fix the samples to the slide? You correctly answered: a. They would all test negative because the washing steps would remove the samples. 3. Which sample showed some residual nonspecific binding after the washing steps? You correctly answered: c. patient C

4. Which patient samples contained the Chlamydia-specific antigen? You
correctly answered: b. patient B
5. How is chlamydia disease transmission prevented?
You correctly answered: d. abstinence or faithful, mutual monogamy

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Review Sheet Results
1. Describe the importance of the washing steps in the direct antibody fluorescence test. Your answer:
needed in order to remove any non specific binding that may have occurred. 2. Explain where the epitope (antigenic determinant) is located. Your answer:
On antigens because they are large and have multiple antigenic sites—locations that can bind to antibodies. 3. Describe how a positive result is detected in this serological test. Your answer:
If there is a presence of 10 or more elementary bodies in a field diameter of 5 milimeters. They will stain green in red host cells.
4. How would the results be affected if a negative control gave a positive result? Your answer:
It would be a “false negative” and it would invalidate my results.

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