Oxidation is the major pathway for the catabolism of saturated fatty acids. It involves the successive removal of two-carbon fragments from the carboxyl end of the fatty acyl CoA (Champe, Harvey, & Ferrier, 2005). The first three steps of this ? -oxidation spiral are the following (Murray, Granner, Mayes, & Rodwell, 2000): Dehydrogenation or oxidation – the removal of two hydrogen atoms from the 2(? )- and 3(? )-carbon atoms, catalyzed by acyl-CoA dehydrogenase, yielding 1 FADH2; Hydration – the addition of water to saturate the double bond, forming 3-hydroxyacyl-CoA, catalyzed by ? 2-enoyl-CoA hydrase (also known as enoyl-CoA hydratase); and Dehydrogenation or oxidation – a further dehydrogenation on the 3-carbon, catalyzed by the enzyme L(+)-3-hydroxyacyl-CoA dehydrogenase, producing 1 NADH….