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Fluorescence Essay Topics & Paper Examples

Determination of Quinine in Tonic Water

i. The selectivity of fluorimetry is greater than that of absorption spectrophotometry as it depends on excitation, absorption and emission spectra. As for fluorimetry, when molecule absorbs radiation and become excited, fluorimetry detects the intensity of light emitted from the molecule returns to ground state from excited state. Fluorescence is good at rigid molecules as it absorbs UV radiation. When two compounds are excited at the same wavelength radiation, they would emit different wavelength radiation as different compound has its unique spectrum. ii. Second, the sensitivity of fluorescence is greater than absorption spectrophotometry. As for absorption spectrophotometry, the concentration is directly proportional to the absorbance. However, in fluorimetry, concentration is directly related to the luminescent radiant power which is measured…