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Bio Lab Microscope Report Essay

The Microscope
Microscope is a tool used to enlarge images of small objects that are hard to study with bare eyes. The compound light microscope, which is going to be used in this lab activity, is an instrument with two lenses and various knobs to focus the image. In this lab, we will learn about the proper use and handling of the microscope. Materials

Compound Microscope
Glass slide with the letter “e” on it
Results

As I viewed the letter, it was orientated upside down. You can’t really see the slide when you move it left or right or toward or away from me. It’s out of your field of view when you move it. When you go from 4x to 10x the brightness of the view dims. When the image is viewed through the high-power objective, the “e” is magnified 400x larger. If you didn’t know what you was looking at and was viewing the image from the high power objective, you wouldn’t know what it was because the higher the magnification the more obscure the slide becomes.

Total Magnification and Areas of Field of View for Three Objectives Objective Power
Objective Magnification

Ocular Magnification

Total Magnification
4x
4x
x
10x
=
40x
10x
10x
x
10x
=
100x
40x
40x
x
10x
=
400x

Conclusion
When handling a light microscope properly, there are two procedures that should be taken carefully. First, we should start observing an object from the low-power objective. To get the image focused when using the low-power objective, we should use coarse adjustment knob to adjust it first, not fine adjustment knob. Fine adjustment knob should be used later, such as when looking at high-power objectives. Otherwise, the slide and the objective can be damaged. Also, we should always treat the microscope with great care. To be specific, the microscope should be carried with two hands; one holding the arm, and another holding the base. Also, when putting down the microscope, it should be placed gently. When not using the microscope, it should be covered with the dust cover and kept. The compound light microscope is the most common microscope that are used in biology classes.

The light microscope is also called compound microscope because it contains two lenses. Unlike a simple microscope that uses one lens, a compound microscope uses more than one lens. As shown in the section of observing an “e”, the images observed under the light microscope are reversed and inverted. This is because when the light passes the lens of the eyepiece, the image gets inverted since those lenses are usually convex lenses. The image is erected again on the retina. The specimen must be centered in the field of view on low power before going to high power because if the specimen is observed on high power from the beginning, it gets very hard to find the specimen. When low-power objective is used from the beginning, we are can start the observation with the entire image of the specimen. Then, it gets much easier to observe the specimen when magnified.


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