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Absorbance Essay Topics & Paper Examples

Analysis of Food Dyes in Beverages

Purpose: To determine the concentration of dye in a sports drink using spectroscopy and graphical analysis. We will be using beers law to find the unknown concentration of dye in a sports drink. Materials: Beakers, 50-mL, 2-3 ; Test tubes (13 x 100)mm 3-8 ; FD&C Blue 1 stock solution, 50-mL ; Lens tissues ; Pipets, serological, 10-mL ; Sample of consumer sports drink, 10-mL ; Spectrophotometer ; Test tube rack Procedure: 1) Preeminently, follow all laboratory safety guidelines ensuring that you have safety goggles on at all times throughout the lab. 2) Fill the cuvette 2/3 of the way with water. 3) Select the appropriate wavelength on the spectrophotometer. 4) Place the cuvette in the spectrophotometer and click the…

Beer’s Law Problem Set

Beer’s Law Problem Set Spring 2013 1. Calculate the absorbances corresponding to the following values of the percentage of transmitted light: (Provide your final answer with three decimal places) a. 95% b. 88% c. 71% d. 50% e. 17.5% f. 1% 2. A solution of a compound (1.0mM) was placed in a spectrophotometer cuvette of light path 1.05cm. The light transmission was 18.4% at 470nm. Determine the molar extinction coefficient. Include units in your answer. 3. The molar extinction coefficient of reduced NADH (nicotinamide adenine dinucleotide phosphophate) at 340nm is 6220 L/mole·cm. 3ml of solution containing 0.2 micromoles of NADH were placed in a cuvette of 1.05cm light path. Calculate the percentage light transmission of this sample at 340nm. 4….