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What organic molecule can be detected with Lugol’s? Essay

When we put the Lugol’s regent (I2Kl) in the beaker, we can observe the effects of osmosis as the Lugol’s regent (I2Kl) solution on the outside of the beaker permeates the intestine (Dialysis tubing) inducing a chemical reaction that will turn the liquid in the intestine (Dialysis tubing) blue-black.

If the amylase successfully digests the starch, what results would you expect? If the amylase successfully digests the starch, I would expect a no color change occur as well as a presence of glucose in the intestine (Dialysis tubing).

If the amylase only partly digests the starch, what would you expect to happen? If the amylase only partly digests the starch, I would expect to see a faint color change as well as a small presence of glucose inside the intestine.

If the amylase fails to digest the starch, what results would you expect? If the amylase fails to digest the starch, I would expect the solution inside the intestine (Dialysis tubing) to change color to blue-black due to the chemical action that will occur when the Lugol’s regent (I2Kl) permeates through the intestine (Dialysis tubing).

Why is it necessary to have a control for this experiment?
By having a control in this experiment, we can see the changes with the presence of amylase. Lugol’s regent (I2Kl) changes color in the presence of starch, which is the control group that we have setup that demonstrates what would happen naturally, with starch and Lugol’s regent (I2Kl). The control group will have a distinct comparison available for us. As amylase, an enzyme that catalyzes the breakdown of the starch into glucose, which will have no reaction with Lugol’s regent (I2Kl) as well as no color change.

Were starch molecules able to cross the membrane?
No, the starch molecules were not able to cross the membrane, because if it were able to pass through, then the solution on the outside of the intestine (Dialysis tubing) would have changed color to blue-black.

Were Lugol molecules able to cross the membrane?
Yes, the starch molecules were able to cross the membrane as we observe the color changes that occur inside the intestine (Dialysis tubing).

What is your conclusion regard amylase digestive activity?
In this experiment, I concluded that amylase have the abilities to catalyze the breakdown of the starch into glucose. Also, some molecules, such as the Lugol’s regent (I2Kl) and glucose are able to permeate through intestine (Dialysis tubing) while others, such as starch is not able to permeate through the (Dialysis tubing).

What does this experiment tell you about the size of glucose molecules and membrane permeability? This experiment tells me the size of the glucose molecule is very small in comparison to starch molecule. They have high membrane permeability that allows them to permeate through the intestine (Dialysis tubing); this is supported by the presence of glucose in the water when we conducted the glucose test.

In the experiment, did the movement of glucose and starch molecule reflect the expected results of biological simulation?

In this experiment, the glucose and starch molecules accurately reflected the expected results of the biological simulation. Animal’s starch molecule can’t cross the plasma membrane to leave the intestine, similarity in our experiment, the starch solution were not able to permeate through the intestine (Dialysis tubing), thus a no change of color occurred in the Lugol’s regent (I2Kl) solution. Furthermore, the glucose molecules can cross membrane between the intestine and circulatory system, and this is proven accurate as we were able to test the presence of glucose in the Lugol’s regent (I2Kl) solution that contain amylase in the intestine
(Dialysis tubing).


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